Journal: International journal of molecular sciences

Article Title: 2-Methoxyestradiol-3,17- O , O -bis-sulfamate (STX140) Inhibits Proliferation and Invasion via Senescence Pathway Induction in Human BRAFi-Resistant Melanoma Cells.

doi: 10.3390/ijms241411314

Figure Lengend Snippet: Figure 4. Anti-invasion activity of STX140 against human melanoma cells. (a) Reconstructed human skin with melanoma micrographs from inverted microscope (20× and 40×, respectively) dyed with hematoxylin/eosin (H&E); (b) Interleukin quantification in media from the 3D reconstructed human skin samples, STX140 (100 nM), results are expressed as the mean of three independent experiments (n = 3) ± S.D.; significance is indicated by *** p < 0.001.

Article Snippet: Interleukins IL-6 (QC193, R&D Systems®, Minneapolis, MN, EUA) and IL-8 (R&D Systems®, AF-208-NA) from samples were quantified by ELISA assay (R&D Systems®) and analyzed according to the instructions of the commercial kit manufacturers.

Techniques: Activity Assay, Inverted Microscopy

Journal: Pediatrics and neonatology

Article Title: The significance of serum and fecal levels of interleukin-6 and interleukin-8 in hospitalized children with acute rotavirus and norovirus gastroenteritis.

doi: 10.1016/j.pedneo.2013.05.008

Figure Lengend Snippet: Figure 1 Serum (A) interleukin-6 (IL-6) and (B) interleukin-8 (IL-8) levels in children with rotavirus, norovirus, bacterial gastroenteritis, and in healthy controls. The median with interquartile range (IQR) are shown in each group. p value for comparisons by Kruskal-Wallis test; *p < 0.05, by Dunn’s mul- tiple comparison test between groups.

Article Snippet: IL-6 and IL-8 levels were measured with a Quantikine Human Interleukin Immuno-assay (R&D Systems, Minneapolis, MN, USA) using the principle of quantitative sandwich enzyme immuno-assay with monoclonal antibodies specific for IL-6 and IL-8.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression

doi: 10.1371/journal.pone.0124865

Figure Lengend Snippet: (A) MMP1, (B) MMP2, (C) MMP3, (D) MMP9 and (E) TIMP1 mRNAs Real-time PCR was used to analyze MT3 mRNA. Relative expression (RQ) of MT3 gene was normalized against expression of ACTB gene and BO2/LUC/PURO cells were assigned as a calibrator sample. Results are expressed as mean ± SD. (F) MMP3 protein level in culture media of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP3. Mann–Whitney test, *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The Quantikine Human Total MMPs Immunoassay (R&D Systems, Minneapolis, MN USA) was used to detect MMP1, MMP2, MMP3, MMP9, and TIMP1 in the cell culture supernatants.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, MANN-WHITNEY

Journal: Central-European Journal of Immunology

Article Title: The diagnostic value of early cytokine response in patients after major trauma – preliminary report

doi: 10.5114/ceji.2018.74871

Figure Lengend Snippet: IL-1Ra concentration (pg/ml) in relation to the extent of the injury (according to the Injury Severity Score [ISS] score). * p < 0.05, group A ISS ≥ 20; group B ISS < 20

Article Snippet: The analysis of cytokine concentrations in peripheral blood plasma was performed on the basis of commercially available kits for ELISA enzymatic immunoassay, in accordance with the instructions provided by the manufacturer (Quantikine Immunoassay human IL-1Ra and human IL-6, R & D Systems Europe, Minneapolis, USA).

Techniques: Concentration Assay

Journal: Central-European Journal of Immunology

Article Title: The diagnostic value of early cytokine response in patients after major trauma – preliminary report

doi: 10.5114/ceji.2018.74871

Figure Lengend Snippet: IL-1Ra concentration [pg/ml] in relation to the complications. * p < 0.05 post-traumatic complications (C + ), without complications (C – )

Article Snippet: The analysis of cytokine concentrations in peripheral blood plasma was performed on the basis of commercially available kits for ELISA enzymatic immunoassay, in accordance with the instructions provided by the manufacturer (Quantikine Immunoassay human IL-1Ra and human IL-6, R & D Systems Europe, Minneapolis, USA).

Techniques: Concentration Assay

Journal: Central-European Journal of Immunology

Article Title: The diagnostic value of early cytokine response in patients after major trauma – preliminary report

doi: 10.5114/ceji.2018.74871

Figure Lengend Snippet: Correlation plot between baseline levels of IL-1Ra and the Injury Severity Score (ISS) scores

Article Snippet: The analysis of cytokine concentrations in peripheral blood plasma was performed on the basis of commercially available kits for ELISA enzymatic immunoassay, in accordance with the instructions provided by the manufacturer (Quantikine Immunoassay human IL-1Ra and human IL-6, R & D Systems Europe, Minneapolis, USA).

Techniques:

Journal: Central-European Journal of Immunology

Article Title: The diagnostic value of early cytokine response in patients after major trauma – preliminary report

doi: 10.5114/ceji.2018.74871

Figure Lengend Snippet: Prognostic value for serum IL-6 and IL-1Ra levels in the whole group of patients after trauma (ROC curve)

Article Snippet: The analysis of cytokine concentrations in peripheral blood plasma was performed on the basis of commercially available kits for ELISA enzymatic immunoassay, in accordance with the instructions provided by the manufacturer (Quantikine Immunoassay human IL-1Ra and human IL-6, R & D Systems Europe, Minneapolis, USA).

Techniques: